This product is cylindrical, conical, oval, or irregular, with a length of 3 to 17 cm and a diameter of 3 to 10 cm. Except for the outer skin, the surface is yellowish brown to reddish brown, and some visible white-like network textures and scattered stars (specially shaped vascular bundles) are scattered. The remaining outer skin is tan, with many rope holes and rough wrinkles. Solid, with a slightly soft center, reddish-brown or yellow-brown in section, showing granularity; the rhizome is broad, with star-point rings or scattered; the roots are well-developed, with a radial texture, with layered rings and no star points . The air is fragrant, bitter and slightly astringent, and the teeth are chewed with a grit.
Cross section of this product: Most of the cork and cortex of the root have been removed. The phloem is obvious; the thin-walled tissue is well developed. The layers form a loop. Xylem has dense rays, 2 to 4 rows of cells, and contains brown objects; the ducts are not woody, often 1 to several clustered together and sparsely arranged. Parenchymatous cells contain clusters of calcium oxalate and most starch granules. The rhizome is broad, with common mucus cavities and reddish-brown objects; the heterotypic vascular bundles are scattered, forming layers to form loops, the xylem is located outside the forming layer, and the bast is located inside the forming layer. Powder yellow-brown. Calcium oxalate clusters have a diameter of 20 to 160 μm, and some to 190 μm. Non-wooded ducts with marginal pits, reticulate, threaded and annular veins. There are many starch grains, single grains are spherical or polygonal, diameter is 3 ～ 45μm, umbilical dots are star-shaped; complex grains are composed of 2-8 grains.
Take a small amount of the powder of this product and perform sublimation, showing rhombus needle crystals or feather crystals.
Take 0.1g of this product powder, add 20ml of methanol for 1 hour, filter, take 5ml of the filtrate, evaporate and add 10ml of water to dissolve, add 1ml of hydrochloric acid, heat in a water bath for 30 minutes, immediately cool, and extract twice with ether. 20 ml each time, the ether solution was combined, evaporated to dryness, and the residue was dissolved by adding 1 ml of chloroform to be used as a test solution. Another 0.1 g of rhubarb reference medicinal material was prepared in the same way as the reference medicinal solution. Then take the rhein reference substance and add methanol to make a solution containing 1mg per 1ml as the control solution. According to the thin-layer chromatography (Appendix VI B) test, 4 μl of each of the above three solutions were pipetted, and respectively spotted on the same silica gel H thin-layer plate with sodium carboxymethyl cellulose as a binder, and petroleum ether (30-60 ° C.) The upper layer solution of) -ethyl formate-formic acid (155: 1) was used as a developing agent, developed, taken out, dried, and inspected under an ultraviolet light (365 nm). In the chromatogram of the test product, the same five main orange-yellow fluorescent spots were displayed at the corresponding positions of the chromatogram of the reference drug; at the corresponding positions of the chromatogram of the reference substance, the same orange-yellow fluorescent spots were displayed, and they were smoked in ammonia vapor. When viewed in daylight, the spots turn red.
Take rhubarb glycoside 0.2g powder, add 2ml of methanol, soak for 10 minutes, let cool, take 10μl of the supernatant, point on filter paper, unfold with 45% ethanol, remove, dry, leave for 10 minutes, put UV When viewed under a light lamp (365nm), no persistent bright purple fluorescence should be displayed.
Take this product after losing weight and dry it at 105 ℃ for 6 hours. The weight loss should not exceed 15.0% (Appendix Ⅸ G).
The total ash content must not exceed 10.0% (Appendix Ⅸ K).
Acid insoluble ash must not exceed 0.8% (Appendix Ⅸ K).
Determined by high performance liquid chromatography (Appendix VI D).
Chromatographic conditions and system suitability tests use octadecylsilane bonded silica as a filler; methanol-0.1% phosphoric acid solution (85:15) as the mobile phase; the detection wavelength is 254nm. The number of theoretical plates should not be less than 1500 based on the emodin peak.
Preparation of reference solution solution Weigh accurately 5mg each of emodin and emodin reference materials, place them in 50ml measuring bottles, dissolve with methanol and dilute to the mark, and shake well; accurately measure 1ml emodin solution and 2ml emodin solution. Put into 25ml measuring flasks respectively, add methanol to the mark, and shake to obtain (Emodin contains 4μg per 1ml, and emodin contains 8μg per 1ml).
Preparation of the solution for the test sample: Take about 0.1g of this product powder (passed through No. 4 sieve) [while taking another sample of this product powder to determine the moisture content (Appendix ⅨH second method)], weigh it accurately, place it in a 50ml conical flask, and add it accurately. 25ml of methanol, weighed, heated to reflux for 30 minutes, allowed to cool, weighed again, make up the lost weight with methanol, shake well, filter, and continue to filter 5ml for precise measurement, place in a 50ml round bottom flask, and wave Methanol, add 10 ml of 2.5 mol / L sulfuric acid solution, sonicate for 5 minutes, add 10 ml of chloroform, heat and reflux for 1 hour, cool, transfer to a separatory funnel, wash the container with a small amount of chloroform, and put it into the separatory funnel, and collect The chloroform layer, the acid solution was extracted twice with chloroform, about 8ml each time, combined with chloroform solution, dehydrated with anhydrous sodium sulfate, the chloroform solution was transferred to a 100ml conical flask, the chloroform was evaporated, and the residue was precisely added with 10ml of methanol and weighed. , Dissolve the residue in a hot water bath, dissolve the residue, let it cool, then weigh it again, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain.
The measurement method accurately sucked 5 μl of each of the two reference solutions and the test solution respectively, injected them into a liquid chromatograph, and measured, and the results were obtained.
This product is calculated on the basis of dry products. The total content of emodin (C15H10O5) and emodin (C15H10O4) should not be less than 0.05%.
Rhubarb removes impurities, washed, moistened, cut into thick pieces or pieces, and dried.
Take wine rhubarb tablets and stir-fry them according to the wine-baking method (Appendix II D).
Take cooked rhubarb nuggets and stew or steam them according to the wine stew or wine steaming method (Appendix II D) until the inside and outside are black.
Take the rhubarb charcoal from the rhubarb charcoal and fry it according to the charcoal frying method (Appendix II D) until the surface is charred and the internal charred.
[Sexual Flavor and Guijing]
Bitter and cold. Spleen, stomach, large intestine, liver, pericardium.
[Function and indication]
Xiere Tongchang, cooling blood detoxification, Zhuyu Tongmeng. For real constipation, stagnation of abdominal pain, diarrhea, uncomfortable diarrhea, hot and humid jaundice, blood-heat vomiting, red eyes, pharyngeal swelling, intestinal cramps and abdominal pain, carbuncle and scabies, stasis and amenorrhea, bruises, external treatment of water and fire ; Upper gastrointestinal bleeding. Wine rhubarb Shanqing on the scorched blood toxin. It is used for red pharyngeal swelling and gingival swelling. Ripe rhubarb diarrhea slows down, purging fire and detoxifying. For fire poison sores. Rhubarb charcoal cools blood and removes blood stasis to stop bleeding. For blood heat and blood stasis.
Health rhubarb has strong diarrhea, so those who want to attack it should use it rawly. The decoction should be used afterwards, or soaked in boiling water; Jiujian will weaken the diarrhea. Rhubarb rhubarb has weak diarrhea, good blood circulation, and easy blood stasis syndrome. Rhubarb charcoal is mostly used for bleeding syndrome [usage and dosage]
3 ～ 30g, it should not be fried for a long time. Appropriate amount for topical application and adjustment at the end of the study [Note]
Use with caution in pregnant women.
Place in a ventilated and dry place to prevent maggots.
Rhubarb flow extract