| [Character] The root of licorice is cylindrical, 25 ～ 100cm long and 0.6 ～ 3.5cm in diameter. The outer skin is elastic. The surface is reddish brown or grayish brown, with prominent vertical wrinkles, grooves, pores, and thin fine root marks. Solid, slightly fibrous in section, |
Yellowish white, powdery, obvious formation of layer rings, radial rays, and some cracks. The rhizome is cylindrical with bud marks on the surface and a pith in the middle of the section. Weiqi, sweet and special.
The roots and rhizomes of Glycyrrhiza inflata are woody and stout, with some branches, rough skins, and grayish brown or grayish brown. It is hard, has many wood fibers, and has low powderiness. Rhizomes have many adventitious buds and are large.
Glycyrrhiza glabra roots and rhizomes are relatively firm in texture, with some branches, the outer skin is not rough, grayish brown, and the pores are thin but not obvious.
[Identification] (1) Cross section of this product: The cork layer is a series of brown cells. The inner layer of the plug is narrow. The phloem has broad rays, many bends, and often cracks; fibers are bundled, non-wooden or micro-woody, and the surrounding thin-walled cells often contain calcium oxalate cubes; the sieve tube group is often deformed by compression. The formation of layers within the bundle is obvious. Xylem rays are 3 to 5 rows of cells; there are many ducts with a diameter of about 160 μm; wood fibers are bundled, and the surrounding thin-walled cells also contain calcium oxalate cubes. Root center without pith; rhizome center with pith.
The powder was light brownish yellow. The fibers are bundled, with a diameter of 8 to 14 μm, wall thickness, and micro-woodification. The surrounding thin-walled cells contain calcium oxalate cubes to form crystalline fibers. Calcium oxalate cubes are common. Bordered reticular ducts are larger, and rare reticulated ducts. The cork cells are reddish brown, polygonal, and slightly woody.
(2) Take 1g of the powder, add 40ml of ether, heat and reflux for 1 hour, filter, discard the ether solution, add 30ml of methanol residue, heat and reflux for 1 hour, filter, evaporate the filtrate, and add 40ml of water to dissolve the residue. Extract three times with 20 ml of n-butanol, combine the n-butanol solution, wash with water three times, discard the water solution, evaporate the n-butanol solution, add 5 ml of methanol to the residue to dissolve, and use it as a test solution. Another 1 g of licorice control medicinal material was prepared, and a control medicinal material solution was prepared in the same way. Then take the glycyrrhizic acid monoammonium salt reference substance and add methanol to make a solution containing 2mg per 1ml as the reference solution. According to the test of “Standard Operating Procedures for Thin-Layer Chromatography Inspection” (Appendix VIB), draw 1-2 μl of each of the above three solutions, and point them on the same silica gel G thin-layer plate prepared with 1% sodium hydroxide solution, and use ethyl acetate. Ester-formic acid-glacial acetic acid-water (15: 1: 1: 2) as the developing agent, unfolded, taken out, air-dried, sprayed with a 10% sulfuric acid ethanol solution, heated at 105 ℃ until the spots became clear, and placed an ultraviolet light (365nm). In the chromatogram of the test product, the fluorescent spots of the same color were displayed at the positions corresponding to the chromatogram of the control medicinal materials; at the corresponding positions of the chromatogram of the reference materials, the same orange-yellow fluorescent spots were displayed.
[Check] Moisture must not exceed 12.0% (Appendix ⅨH First Method).
The total ash content must not exceed 7.0% (Appendix ⅨK).
Acid insoluble ash must not exceed 2.0% (Appendix ⅨK).
Determination of heavy metals and harmful elements according to lead, cadmium, arsenic, mercury, and copper (Appendix ⅨA Atomic Absorption Spectrophotometry or Inductively Coupled Plasma Mass Spectrometry). Lead must not exceed 5 parts per million; cadmium must not exceed 10 parts per million. 3. Arsenic must not exceed 2 parts per million; mercury must not exceed 2 parts per million; copper must not exceed 20 parts per million.
Organochlorine pesticide residues are determined according to the pesticide residue determination method (Appendix ⅨQ Organochlorine pesticide residues determination). The quantity of 666 (total BHC) must not exceed two ten-thousandths; DDT (total DDT) must not exceed ten-thousandths. Two; Pentachloronitrobenzene (PCNB) must not exceed one ten million.
[Content determination] Determine according to "High Performance Liquid Chromatography Test Standard Operating Procedures".
Chromatographic conditions and system suitability test: Octadecylsilane bonded silica as filler, acetonitrile as mobile phase A, 0.05% phosphoric acid solution as mobile phase B, gradient elution according to the regulations in the table; detection wavelength It is 237 nm. The number of theoretical plates should not be less than 5,000 based on the glycyrrhizin peak.
Time (minutes) Mobile phase A (%) Mobile phase B (%)
0 ~ 81981
8 ~ 3519 ~ 5081 ~ 50
35 ~ 3650 ~ 10050 ~ 0
36 ~ 40100 ~ 190 ~ 81
Preparation of reference solution: Take appropriate amounts of glycyrrhizin reference and ammonium glycyrrhizin reference, accurately weighed, and add 70% ethanol to make a solution containing 20 μg of glycyrrhizin and 0.2 mg of glycyrrhizin per 1 ml, respectively. Monoglycyrrhizinate weight / 1.0207).
Preparation of test solution Take about 0.2g of this product powder (passed through No. 3 sieve), weigh it accurately, place it in a conical flask with stopper, add 100ml of 70% ethanol, tightly, weigh it, and sonicate ( Power (250W, frequency: 40kHz) for 30 minutes, let cool, weigh again, make up the lost weight with 70% ethanol, shake well, filter, and take the filtrate to obtain.
Assay method: precision draw the reference solution and test solution
10 μl was injected into a liquid chromatograph and measured to obtain it.
This product is calculated on the basis of dry products. The content of glycyrrhizin (C21H22O9) should not be less than 0.50%, and the glycyrrhizic acid (C42H62O16) should not be less than 2.0%.
Pieces [prepared] to remove impurities, wash, moisten, cut into thick pieces, and dry.
[Sexual flavor and guijing] sweet, flat. Return to Heart, Lung, Spleen, and Stomach Meridian.
[Functions and Indications] Nourish the spleen and replenish qi, clear heat and detoxify, expectorant and cough, relieve pain and analgesia, and reconcile various medicines. It is used for weak spleen and stomach, fatigue, fatigue, shortness of breath, cough and sputum, abdomen and extremity cramps and pain, carbuncle and sore, relieve drug toxicity and severeness.
[Usage and dosage] 2 ～ 10g.
[Note] It should not be used together with seaweed, Euphorbia pulcherrima, Red Euphorbia, Gansui, Lihua.
[Storage] Store in a ventilated and dry place to prevent moth.